Revista argentina de microbiología
versión On-line ISSN 1851-7617
PINONI, M.V. et al. Useful phenotypic characteristics for presumptive identification of Candida guilliermondii. Rev. argent. microbiol. [online]. 2007, vol.39, n.2, pp. 81-83. ISSN 1851-7617.
Candida guilliermondii developed a pink-purplish colony on CHROMagar Candida. In the micromorphology in milk-tween 80 1% agar at 28 °C after 48 h of incubation C. guilliermondii showed small (3-5 µm), spherical yeasts without pseudohyphaes. This Candida species presented a characteristic cluster of blastospores with pseudohyphaes radiating from the centre at 96 h. The trehalose-sucrose assimilation assay was applied to the C. guilliermondii isolates which proved negative for trehalose and positive for sucrose. These results allowed for the presumptive identification of C. guilliermondii. The results were concordant in 100% of the isolates with the identification of the C. guilliermondii species by the ID 32C and Vitek YBC methods. Such automated methods offered Candida famata as a second option, with a reliability percentage of 10%. Micromorphological studies increase yeast identification reliability, especially among species presenting similar biochemical profiles.
Palabras clave : Candida guilliermondii; Candida micromorphology; CHROMagar Candida; Candida.