Abstract

ROLDAN, M. L. et al. Isolation, characterization and typing of Escherichia coli O157:H7 strains from beef products and milk. Rev. argent. microbiol. [online]. 2007, vol.39, n.2, pp.113-119. ISSN 0325-7541.

Shiga toxin (Stx)-producing Escherichia coli (STEC) is an emergent pathogen associated with foodborne diseases, especially foodstuffs of animal origin. A total of 250 beef samples (ground beef and hamburgers) obtained from retail outlets in Santa Fe and Santo Tomé cities, and 150 milk samples from bulk tank milk from dairy barns of the region were analyzed by selective enrichment and immunomagnetic separation. Escherichia coli O157:H7 stx2, eae and ehxA positive strains were isolated from three (1.2%) beef samples. The strains could be differentiated by pulsed-field gel electrophoresis, phagetyping and genotyping of stx. The milk samples were negative for STEC O157. These findings confirm the role of food of animal origin in the epidemiology of E. coli O157:H7 - associated diseases.

Keywords : Escherichia coli O157:H7; Meat products; Milk.

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