SciELO - Scientific Electronic Library Online

 
vol.39 issue4Simplified prediction of Staphylococcus aureus growth in a cooked meat product exposed to changing environmental temperatures in warm climates author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Revista argentina de microbiología

On-line version ISSN 1851-7617

Abstract

ARA-ROJAS, S. L.  and  MASSOL-DEYA, A.. Bacterial diversity in a fluidized bed bioreactor (FBR) treating gasoline-contaminated groundwater. Rev. argent. microbiol. [online]. 2007, vol.39, n.4, pp. 243-251. ISSN 1851-7617.

The main objective of this research project was to determine the bacterial diversity during the process of bioremediation of water contaminated with gasoline in a fluidized bed reactor at Mayagüez, PR. Isolation and characterization of bacterial populations from the bioremediation system was performed on R2A medium. Morphological tests included cellular and colonial shape and reaction to Gram coloration. Physiological properties were determined by using carbon utilization profiles (Biolog®) and by the ability of axenic cultures to use gasoline as the sole carbon source. Molecular characterization was performed by BOX-PCR and 16S rDNA sequence analysis (ARDRA). From a total of 162 distinctive isolates, 75% were gram-negative bacilli, 19% gram-positive bacilli, 5% gram-negative cocci and 1% gram-positive cocci. The 162 axenic cultures corresponded to 90 different genetic groups; 53% of which included strains with growth in gasoline as sole carbon source. The 86 strains capable of growing in gasoline corresponded to 52 different amplification patterns in BOX-PCR; which were not metabolically related (Biolog® system). The high degree of microbial diversity in the FBR allowed efficient and stable hydrocarbon removal throughout the operation of the system.

Keywords : Bioremediation; Gasoline biodegradation; Bacteria; BOX-PCR; ARDRA.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License