Revista argentina de microbiología
versión On-line ISSN 1851-7617
REYES-MONTES, M. R. et al. Identification of the source of histoplasmosis infection in two captive maras (Dolichotis patagonum) from the same colony by using molecular and immunologic assays. Rev. argent. microbiol. [online]. 2009, vol.41, n.2, pp. 102-104. ISSN 1851-7617.
Histoplasma capsulatum was isolated from the spleen of a first infected mara (Dolichotis patagonum) and from a second mara's liver and adrenal gland, both in the same colony at the Africam Safari, Puebla, Mexico. Studies of H. capsulatum isolates, using nested-PCR of a 100-kDa protein coding gene (Hcp100) fragment and a two-primer RAPDPCR method, suggest that these isolates were spreading in the environment of the maras' enclosure. By using a Dot- ELISA method, sera from mice inoculated with three homogenates of soil samples from the maras' enclosed space developed positive brown spot reactions to a purified H. capsulatum antigen, which identified the probable source of the maras' infection.
Palabras llave : Histoplasma capsulatum; Maras; PCR; RAPD-PCR; Dot-ELISA; Infection source.