SciELO - Scientific Electronic Library Online

vol.41 número4Brote de micoplasmosis clínica por Mycoplasma ovis en ovinos de Salta, Argentina: Diagnóstico clínico, microbiológico y molecular índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados



  • No hay articulos citadosCitado por SciELO

Links relacionados


Revista argentina de microbiología

versión On-line ISSN 1851-7617


SGUAZZA, G. H. et al. Complete genome amplification of Equine influenza virus subtype 2. Rev. argent. microbiol. [online]. 2009, vol.41, n.4, pp. 207-211. ISSN 1851-7617.

This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScriptTM reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a complex secondary structure. The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies.

Palabras clave : Equine influenza virus; Genome; Diagnosis; RT-PCR.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License