Abstract

SALARIATO, D.; DIORIO, L. A.; MOUSO, N.  and  FORCHIASSIN, F.. Extraction and characterization of polygalacturonase of Fomes sclerodermeus produced by solid-state fermentation. Rev. argent. microbiol. [online]. 2010, vol.42, n.1, pp.57-62. ISSN 0325-7541.

Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na₂SO₄ as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained.

Keywords : Polygalacturonase; Solid state fermentation; Fomes sclerodermeus.

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