Revista argentina de microbiología
versión On-line ISSN 1851-7617
SALARIATO, D.; DIORIO, L. A.; MOUSO, N. y FORCHIASSIN, F.. Extraction and characterization of polygalacturonase of Fomes sclerodermeus produced by solid-state fermentation. Rev. argent. microbiol. [online]. 2010, vol.42, n.1, pp. 57-62. ISSN 1851-7617.
Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained.
Palabras clave : Polygalacturonase; Solid state fermentation; Fomes sclerodermeus.