SciELO - Scientific Electronic Library Online

 
vol.42 número1Actividad in vitro de la tigeciclina en aislamientos clínicos de Acinetobacter spp. multirresistentes: Comparación de diferentes métodos de evaluaciónIntegrones: los coleccionistas de genes índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

Compartilhar


Revista argentina de microbiología

versão impressa ISSN 0325-7541versão On-line ISSN 1851-7617

Resumo

SALARIATO, D.; DIORIO, L. A.; MOUSO, N.  e  FORCHIASSIN, F.. Extraction and characterization of polygalacturonase of Fomes sclerodermeus produced by solid-state fermentation. Rev. argent. microbiol. [online]. 2010, vol.42, n.1, pp.57-62. ISSN 0325-7541.

Polygalacturonase (PG) production by Fomes sclerodermeus using solid-state fermentation (SSF) was carried out. Maximal PG activity (26 U/gdw) was obtained between days 11 and 13 at the end of exponential growth. PG activity in the crude extract was more stable at pH 5-6 and 30 °C and had optimum activity at pH 5 and 50 °C. Optimal conditions for PG extraction were: one time extraction with Na2SO4 as solvent with 10 min. of agitation. In a scale-up system, PG activity per gram of dry substrate decreased about 60% compared with the activity obtained in an Erlenmeyer flask; however, high total PG activity was obtained.

Palavras-chave : Polygalacturonase; Solid state fermentation; Fomes sclerodermeus.

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons