Revista argentina de microbiología
versión On-line ISSN 1851-7617
TREJO, F. M. et al. Comparison of diagnostic methods for Clostridium difficile-associated diarrhea. Rev. argent. microbiol. [online]. 2010, vol.42, n.3, pp. 165-171. ISSN 1851-7617.
In order to compare different methods for the diagnosis of Clostridium difficile-associated diarrhea, fecal filtrates from patients presenting symptoms compatible with this condition, were analyzed. Biological activity on Vero cells (biological assay), dot blot with antibodies anti-TcdA and anti-TcdB, and a PCR assay for the tcdB gene, were evaluated. Titles of biological assays were ≥ 64 for 44 out of 177 samples. Nineteen samples were positive in both biological and PCR assays. The analysis by dot blot using anti-TcdA and anti-TcdB antibodies showed that 46 samples out of 149 were positive for both toxins whereas 12 samples were only positive for TcdB, and 5 samples only positive for TcdA. Discrepancies in the different methods could be related to truncated genes, low number of microorganisms in the samples and toxin degradation. The results herein presented show the need for developing diagnostic approaches compatible with the complex epidemiological situation of this clinically relevant intestinal pathogen.
Palabras clave : Clostridium difficile; Diagnostic; PCR; Dot blot; Vero cells.