Revista argentina de microbiología
versão impressa ISSN 0325-7541
DEGESE, María F et al. Contribution of the PCR assay to the diagnosis of Mansonella ozzardi in endemic areas of Argentina. Rev. argent. microbiol. [online]. 2012, vol.44, n.2, pp. 97-100. ISSN 0325-7541.
Mansonella ozzardi is a tissue-dwelling parasitic nematode, the causative agent of mansonelliasis in almost all Latin American countries. It has been described along the Argentine Yungas region. The microscopic diagnosis can yield false-negative test results at low microfilaremia levels. The aim of this study was to optimize the molecular diagnostic technique and compare it with the Knott's method and standard blood smear procedures (thin blood films and thick smears) in 92 blood samples of individuals from an endemic area. The PCR technique followed by the sequencing of the amplified product yielded 100 % sensitivity compared to the Knott's test, which is considered a reference method. Seven more cases of this parasitosis could only be identified with the molecular technique.
Palavras-chave : Mansonella ozzardi; Diagnosis; PCR; Argentina.