Revista argentina de microbiología
versión ISSN 0325-7541
LEVIN, Laura; DIORIO, Luis; GRASSI, Emanuel y FORCHIASSIN, Flavia. Grape stalks as substrate for white rot fungi, lignocellulolytic enzyme production and dye decolorization. Rev. argent. microbiol. [online]. 2012, vol.44, n.2, pp. 105-112. ISSN 0325-7541.
The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58 % for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xylidine, respectively, in 5 h.
Palabras llave : Grape stalks; Solid state fermentation; White rot fungi; Lignocellulolytic enzymes; Dye degradation.