Abstract

TOGNERI, Ana M et al. Dissemination of bla_IMP-8 among Enterobacteriaceae isolates from a Buenos Aires Hospital. Rev. argent. microbiol. [online]. 2013, vol.45, n.2, pp.104-109. ISSN 0325-7541.

From August 2008 to December 2011, six metallo-ß-lactamase-producing isolates, four Enterobacter cloacae, one Klebsiella oxytoca and one Citrobacter freundii, were detected at Hospital Interzonal General de Agudos "Evita" in Lanús. All six isolates showed multiresistant profiles and the presence of the bla_IMP-8 gene. Five isolates also expressed PER-2 extended spectrum ß-lactamase. The bla_IMP-8 gene was found as the first cassette in a class 1 integron. However, the 3´ conserved sequence could not be detected in three isolates. In all cases, bla_IMP-8 was transferred by conjugation to azide-resistant Escherichia coli J53. PFGE analysis revealed that the four E. cloacae isolates were not genetically related. These are the first metallo-ß-lactamases detected in this institution and our results suggest a possible intra- and inter-species horizontal dissemination of bla_IMP-8.

Keywords : Metallo-ß-lactamases; Enterobacteria; bla_IMP-8.

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