SciELO - Scientific Electronic Library Online

vol.45 issue3Arsenic tolerance of cyanobacterial strains with potential use in biotechnologyEmergence of colistin-resistant Klebsiella pneumoniae: microbiological and epidemiological characterization of the isolates producing and non-producing KPC-type carbapenemase author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links


Revista argentina de microbiología

Print version ISSN 0325-7541


FERNANDEZ-RENDON, Carlos L  and  BARRERA-ESCORCIA, Guadalupe. Comparison of techniques for coliform bacteria extraction from sediment of Xochimilco Lake, Mexico. Rev. argent. microbiol. [online]. 2013, vol.45, n.3, pp.180-184. ISSN 0325-7541.

The need to separate bacteria from sediment in order to appropriately count them has led to test the efficacy of different techniques. In this research, traditional techniques such as manual shaking, homogenization, ultrasonication, and surfactant are compared. Moreover, the possibility of using a set of enzymes (pancreatine) and an antibiotic (ampicillin) for sediment coliform extraction is proposed. Samples were obtained from Xochimilco Lake in Mexico City. The most probable number of coliform bacteria was determined after applying the appropriate separation procedure. Most of the techniques tested led to numbers similar to those of the control (manual shaking). Only with the use of ampicillin, a greater total coliform concentration was observed (Mann-Whitney, z = 2.09; p = 0.03). It is possible to propose the use of ampicillin as a technique for total coliform extraction; however, it is necessary to consider sensitivity of bacteria to the antibiotic.

Keywords : Separation of bacteria; Glycocalyx; Most probable number.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License