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Revista argentina de microbiología

On-line version ISSN 1851-7617


ALIPPI, Adriana M.; REYNALDI, Francisco J.  and  LOPEZ, Ana C.. Evaluation of the Epsilometer (Etest) method for the detection of tetracycline susceptibility in Paenibacillus larvae, the causal agent of American foulbrood disease of honeybees. Rev. argent. microbiol. [online]. 2013, vol.45, n.4, pp.257-261. ISSN 1851-7617.

American foulbrood (AFB) is a bacterial disease caused by the spore-forming, grampositive bacterium Paenibacillus larvae, which affects honeybee broods worldwide. The aim of this work was to compare the Epsilometer test (Etest) to the agar dilution method for testing a collection of 22 P. larvae strains to tetracycline by using MYPGP and Iso- Sensitest agars. Results showed that a categorical agreement of 100% was found when using Iso-Sensitest, while a categorical agreement of 86.36% was found (with 3 minor errors) when MYPGP was tested. In conclusion, the Etest could be a rapid and reliable method for testing MIC values of tetracycline in P. larvae only when used in combination with Iso-Sensitest agar. Nevertheless, these results should be confirmed with future studies involving a larger number of isolates.

Keywords : Paenibacillus larvae; American foulbrood; Honeybees; Tetracycline; Etest; Antibiotic resistance.

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