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Revista argentina de microbiología

versión On-line ISSN 1851-7617


ALIPPI, Adriana M.; REYNALDI, Francisco J.  y  LOPEZ, Ana C.. Evaluation of the Epsilometer (Etest) method for the detection of tetracycline susceptibility in Paenibacillus larvae, the causal agent of American foulbrood disease of honeybees. Rev. argent. microbiol. [online]. 2013, vol.45, n.4, pp.257-261. ISSN 1851-7617.

American foulbrood (AFB) is a bacterial disease caused by the spore-forming, grampositive bacterium Paenibacillus larvae, which affects honeybee broods worldwide. The aim of this work was to compare the Epsilometer test (Etest) to the agar dilution method for testing a collection of 22 P. larvae strains to tetracycline by using MYPGP and Iso- Sensitest agars. Results showed that a categorical agreement of 100% was found when using Iso-Sensitest, while a categorical agreement of 86.36% was found (with 3 minor errors) when MYPGP was tested. In conclusion, the Etest could be a rapid and reliable method for testing MIC values of tetracycline in P. larvae only when used in combination with Iso-Sensitest agar. Nevertheless, these results should be confirmed with future studies involving a larger number of isolates.

Palabras clave : Paenibacillus larvae; American foulbrood; Honeybees; Tetracycline; Etest; Antibiotic resistance.

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