Abstract

TAMIOZZO, Pablo J et al. Detection of Mycoplasma canadense and Mycoplasma californicum in dairy cattle from Argentina. Rev. argent. microbiol. [online]. 2014, vol.46, n.2, pp.119-121. ISSN 0325-7541.

Different species of Mycoplasma can affect bovine cattle, causing several diseases. PCR sequencing and further analysis of the 16S-23S rRNA ITS region have shown a significant interspecies variability among Mollicutes. Sixteen suspected isolates of Mycoplasma spp. obtained from milk samples from dairy herds were amplified (16S-23S rRNA ITS region). Fourteen out of those 16 suspected Mycoplasma spp. isolates were PCR-positive. To confirm the identity of Mycoplasma bovis, these 14 isolates were tested by another species-specific PCR. Seven of the isolates rendered a positive result. The products of 16S-23S rRNA ITS PCR from one isolate that was identified as M. bovis and from two other isolates, identified as non- M. bovis were randomly selected, sequenced and analyzed. The three sequences (A, B and C) showed 100% similarity with M. bovis, Mycoplasma canadense and Mycoplasma californicum respectively.

Keywords : Dairy cattle; Mycoplasma bovis; Mycoplasma canadense; Mycoplasma californicum; ITS; 16S-23S rRNA.

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