Services on Demand
Journal
Article
Indicators
- Cited by SciELO
Related links
- Similars in SciELO
- uBio
Share
Revista argentina de microbiología
Print version ISSN 0325-7541
Abstract
KUHAR, Francisco and PAPINUTTI, Leandro. Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers. Rev. argent. microbiol. [online]. 2014, vol.46, n.2, pp.144-149. ISSN 0325-7541.
Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and ferulic acid are the best laccase inducers. It was also observed that the two types of inducers (phenolic and metallic) produce different electrophoretic patterns of laccase activity. Optimized concentrations of inducers were obtained through a factorial design and the thermal stability of optimized supernatants was studied at a wide range of acidic pH. We found that the enzyme is more thermostable at higher pH values.
Keywords : Ganoderma; Laccase; Induction; Optimization; Phenolic compounds; Copper.