SciELO - Scientific Electronic Library Online

 
vol.46 issue2Mycotrophic capacity and efficiency of microbial consortia of arbuscular mycorrhizal fungi native of soils from Buenos Aires province under contrasting managementQuantification of parasites in aquatic environments in the Province of Salta, Argentina author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Revista argentina de microbiología

Print version ISSN 0325-7541

Abstract

KUHAR, Francisco  and  PAPINUTTI, Leandro. Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers. Rev. argent. microbiol. [online]. 2014, vol.46, n.2, pp.144-149. ISSN 0325-7541.

Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and ferulic acid are the best laccase inducers. It was also observed that the two types of inducers (phenolic and metallic) produce different electrophoretic patterns of laccase activity. Optimized concentrations of inducers were obtained through a factorial design and the thermal stability of optimized supernatants was studied at a wide range of acidic pH. We found that the enzyme is more thermostable at higher pH values.

Keywords : Ganoderma; Laccase; Induction; Optimization; Phenolic compounds; Copper.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License