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Revista argentina de microbiología

Print version ISSN 0325-7541


GOMEZ, Lucía P et al. Sensitivity of the COBAS AmpliScreenTMHIV-1 Test v1.5 for HIV-1 detection. Rev. argent. microbiol. [online]. 2014, vol.46, n.3, pp.196-200. ISSN 0325-7541.

The introduction of nucleic acid amplification techniques (NAT) in blood banks was intended to reduce the residual risk of transfusion-transmitted infections. Co-circulation of a great diversity of HIV-1 variants in Argentina portrays the need to assess the sensitivity of serological and molecular assays available for their detection. In this study, we evaluated the sensitivity of the COBAS AmpliScreenTMHIV-1 Test, version 1.5 (Roche) for the detection of HIV-1 RNA in plasma samples of infected individuals from Argentina. The results of this study reveal that this technique has high sensitivity for the detection of HIV-1 RNA under assay conditions: using mini-pool testing, pools = 50 RNA copies per ml achieved = 92 % sensitivity, whereas in the standard procedure, samples = 207 RNA copies/ ml achieved 100 % sensitivity. Moreover, the COBAS AmpliScreenTMHIV-1 Test, version 1.5 (Roche) is suitable for detecting prevailing HIV-1 variants.

Keywords : NAT; HIV-1; OBAS AmpliScreen.

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