Abstract

CARBONARI, Claudia C et al. First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina. Rev. argent. microbiol. [online]. 2014, vol.46, n.4, pp.302-306. ISSN 0325-7541.

We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx₁ and stx₂). A mPCR to detect the rfb_O104, fliC_H4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpf_O113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpf_O113, rfb_O104, fliC_H4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes.

Keywords : Enteroaggregative Escherichia coli; O104:H4 serotype; Diarrhea.

        · abstract in Spanish     · text in English     · English ( pdf )