Resumen

ALMEIDA, Isabela Neves de; ALEIXO, Agdemir Valéria; CARVALHO, Wânia da Silva  y  MIRANDA, Silvana Spindola de. In-house PCR with DNA extracted directly from positive slides to confirm or exclude the diagnosis of tuberculosis: focus on biosafety. Rev. argent. microbiol. [online]. 2015, vol.47, n.1, pp.47-49. ISSN 1851-7617.  http://dx.doi.org/10.1016/j.ram.2014.12.002.

The possibility to obtain DNA from smears is a valuable alternative to remedy the lack of samples when they are totally used for bacilloscopy; this technique solves the biosafety problem related to a possible accident with the transportation of flasks containing potentially transmissible clinical samples. Hence, the purpose of this study was to utilize the insertion sequence IS6110 for amplification of DNA from a smear-positive sample for tuberculosis (TB) diagnosis. Among the 52 positive bacilloscopies, sensitivity, specificity, positive predictive value and negative predictive value were 52.3 %, 100 %, 100 % and 89.7 %, respectively whereas accuracy was 90.7 %. The IS6110-based PCR for TB diagnosis developed in DNA extracted from a positive smear is a fast, simple, specific, and safe method.

Palabras clave : M; tuberculosis; Slides; IS6110-PCR; Tuberculosis; Biosafety.

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