Abstract

JURE, María A et al. Isolation and characterization of Escherichia coli O157 in bovine meat products and cattle in the province of Tucuman. Rev. argent. microbiol. [online]. 2015, vol.47, n.2, pp.126-131. ISSN 1851-7617.  http://dx.doi.org/10.1016/j.ram.2015.03.006.

Escherichia coli O157 is an emergent pathogen associated with diarrhea, hemorrhagic colitis and hemolytic uremic syndrome. Meat products constitute an important transmission source of this microorganism. The aims of this study were to characterize E. coli O157 isolated from cattle and meat products collected from abattoirs and retail stores, to establish the clonal relatedness among regional isolates and to compare them with those in the national database. Between 2004 and 2013, 169 minced meat, 35 sausage and 216 carcass samples were analyzed. Thirteen E. coli O157 isolates were identified; 6 of which were O157:H7 and characterized as stx_2c(vh-a)/eae/ehxA (n = 5) and stx₂/eae/ehxA (n = 1). The 7 remaining isolates were non-toxigenic E. coli strains, and serotyped as O157:NT (n = 4), O157:NM (n = 1), O157:ND (n = 1) and O157:H16 (n = 1). The strains yielded different XbaI-PFGE patterns. Compared to the E. coli O157 isolates in the National Database, none of these patterns have been previously detected in strains of different origin in Argentina.

Keywords : Escherichia coli O157; Molecular characterization; Meat products; Carcasses.

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