SciELO - Scientific Electronic Library Online

 
vol.47 issue3Efficiency of bacteriological culture and the immunofluorescent assay to detect Campylobacter fetus in bovine genital fluidsDiphyllobothrium sp. in Canis familiaris from the subtropical area of Argentina (Puerto Iguazú, Misiones) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Revista argentina de microbiología

Print version ISSN 0325-7541

Abstract

POSSE, Tamara; HERMES, Ricardo L.  and  KAUFMAN, Sara C.. Rapid identification of microorganisms by mass spectrometry in a blood culture system: Comparison of two procedures. Rev. argent. microbiol. [online]. 2015, vol.47, n.3, pp.190-195. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2015.06.001.

Rapid identification of microorganisms is critical in hospitalized infected patients. Blood culture is currently the gold standard for detecting and identifying microorganisms causing bacteremia or sepsis. The introduction of mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS) in microbiology laboratories, especially in microorganisms growing in blood culture bottles, provides rapid identification. This study evaluates the performance of the Maldi Sepsityper Biotyper procedure (hereinafter, MS) compared to that of an in-home method (hereinafter, HF). Eight hundred and forty (840) positive blood culture bottles were processed using the HF procedure, 542 of which were also processed using MS. The organisms were identified in 670 (79. 76 %) and 391 (72. 14 %) bottles respectively (p = 0,0013). This study demonstrates the effectiveness of both procedures for identifying microorganisms directly from positive blood culture bottles. However, the HF procedure proved to be more effective than MS, especially in the presence of Gram positive organisms.

Keywords : MALDITOF MS; Blood culture; Rapid methods; Identification.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License