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Revista argentina de microbiología
versión impresa ISSN 0325-7541versión On-line ISSN 1851-7617
Resumen
SUSEVICH, María L; METZ, Germán E; MARTI, Gerardo A y ECHEVERRIA, María G. Dicistroviridae: A new viral purification technique. Rev. argent. microbiol. [online]. 2017, vol.49, n.4, pp.311-314. ISSN 0325-7541. http://dx.doi.org/10.1016/j.ram.2017.04.008.
The family Dicistroviridae comprises three genera and about twenty species of RNA virus, most of them with health or agricultural importance. The Triatoma virus (TrV) is the only entomopathogenic virus identified in triatomine bugs up to the present. TrV replicates within the intestinal epithelial cells, causing high mortality rate and delayed development of the molt of these bugs. TrV has been proposed as a biological control agent for vectors of Chagas disease. Viral particles were purified from feces of 1, 5 and 10 insects from an experimental colony of Triatoma infestans infected with TrV. Viral concentration and infectivity were corroborated using polyacrylamide gels and RT-PCR, respectively. In this work we report a method of viral purification that allows to reduce necessary reagents and time, using a very small amount of fecal matter.
Palabras clave : Dicistrovirus; RNA virus; Viral purification; Triatomines.