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Revista argentina de microbiología
Print version ISSN 0325-7541On-line version ISSN 1851-7617
Abstract
RODRIGUEZ ACOSTA, Fátima et al. Análisis MLVA y perfil de virulencia de aislamientos de Staphylococcus aureus resistentes a la meticilina adquiridos en la comunidad causantes de infecciones pediátricas en Paraguay. Rev. argent. microbiol. [online]. 2018, vol.50, n.2, pp.151-156. ISSN 0325-7541. http://dx.doi.org/10.1016/j.ram.2017.03.012.
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is one of the first causes of skin and soft tissue infections, and can also produce severe diseases such as osteomyelitis and pneumonia. The aim of this descriptive study was to determine the SCCmec type and virulence profile and to study the genetic diversity by MLVA analysis of 21 CA-MRSA isolates that infected Paraguayan children in 2010. The SCCmec type and virulence factors were performed by PCR and genetic diversity by MLVA (multiple locus variable analysis). All the isolates carried SCCmec cassette IV. hla, hlb and sea genes were detected in 28,6%, 9,5% and 4,8% respectively. The MLVA analysis showed high genetic diversity with congruent antibiotic resistance and virulence profiles. This study revealed the presence of CA-MRSA harbouring SCCmec IV with high genetic diversity, providing information not available in our country.
Keywords : Methicillin-resistant Staphylococcus aureus; Children; Virulence factors; Molecular typing.