Abstract

CACERES, María E; ETCHEVERRIA, Analía I  and  PADOLA, Nora L. Effects of the culture medium and the methodology applied on the biofilm formation of 2 diarrheagenic Escherichia coli strains. Rev. argent. microbiol. [online]. 2019, vol.51, n.3, pp.208-213. ISSN 0325-7541.  http://dx.doi.org/10.1016/j.ram.2018.04.007.

The ability to form biofilms of pathogenic microorganisms in a wide variety of environments, surfaces and conditions constitute an important risk, both for the food industry and for public health. The aim of this work was to evaluate and to compare the effects of the methodology applied and the culture medium used on the ability of a non-O157 verotoxigenic Escherichia coli strain and an enteropathogenic strain to form biofilm on polystyrene surface. Two methodological variants were tested in static culture and culture mediums with different composition were used. The results showed that both strains were able to form a greater biofilm under culture in LB supplemented with glucose, with medium replacement at 24 h and the quantification of the biofilm carried out at 48 h of incubation. These conditions could be used in future studies on biofilm formation.

Keywords : Verotoxigenic Escherichia coli; Enteropathogenic Escherichia coli; Biofilm; Methodology; Culture medium.

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