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Revista argentina de microbiología
Print version ISSN 0325-7541On-line version ISSN 1851-7617
Abstract
PIDRE, Matías Luis; SABALETTE, Karina Belén; ROMANOWSKI, Víctor and FERRELLI, María Leticia. Identification of an Argentinean isolate of Spodoptera frugiperda granulovirusIdentificación de un aislamiento argentino de Spodoptera frugiperda granulovirus. Rev. argent. microbiol. [online]. 2019, vol.51, n.4, pp.381-385. ISSN 0325-7541. http://dx.doi.org/10.1016/j.ram.2018.10.003.
The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is an important maize pest. Due to the environmental impact and emergence of resistance caused by chemical pesticides and transgenic events, the use of baculoviruses becomes a safe and useful alternative for its control in integrated pest management strategies. Here we report the identification of a novel isolate of a granulovirus of S. frugiperda native to the central region of Argentina, named SfGV ARG. We observed that larvae infected with SfGV ARG showed a yellowish coloration, swollen body and, in some cases, severe lesions in the last abdominal segments. We confirmed the identity of the isolate by sequencing fragments of the lef-8, lef-9 and granulin genes and by calculating evolutionary distances using the Kimura-2-Parameter model. SfGV ARG DNA restriction pattern allowed to estimate a genome of at least 135 kb.
Keywords : Oruga militar tardía; SfGV; Plaga agrícola; Control biológico; Baculovirus.