Abstract

SALVO, Luciana P. Di et al. The drop plate method as an alternative for Azospirillum spp viable cell enumeration within the consensus protocol of the REDCAI network. Rev. argent. microbiol. [online]. 2022, vol.54, n.2, pp.61-70. ISSN 0325-7541.

Quality evaluation of commercial inoculants is essential to warrant an adequate cropresponse to inoculation within a biosecurity framework. In this sense, this work is aimed at standardizing and validating the drop plate method for the enumeration of Azospirillum viable cellsas an alternative to the spread plate technique, which is currently proposed in the consensusprotocol of the REDCAI network. Between 14 and 25 private and public laboratories partici-pated in three independent trials. We obtained consistent and robust results that allowed toconfirm that both techniques are equivalent, concluding that the drop plate method is an alternative enumeration technique that is adequate to be included in the abovementioned consensusprotocol.

Keywords : PGPR; Quality evaluation; Microbiological procedures; Spread platetechnique; Bioinputs.

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