Abstract

NEDER, Verónica E.; AMADIO, Ariel F.  and  CALVINHO, Luis F.. Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina. Rev. argent. microbiol. [online]. 2022, vol.54, n.2, pp.101-110. ISSN 0325-7541.

There is scarce information about the frequency and epidemiological and clinicalfeatures associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objec-tives of this study were to develop a multiplex PCR for identifying M. bovis and M. canadenseand to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to adiagnostic laboratory. Of a total of 1548 samples from intramammary infections, bulk tank milkand biological fluids, 38 Mycoplasma isolates were obtained. M. bovis, M. canadense, M. cali-fornicum and M. leachii were detected by using two multiplex PCRs, confirming their presencein clinical conditions in dairy cattle. The techniques used in the present study can be usefulto broaden the knowledge about Mycoplasma infections in cattle, since the search for theseorganisms is not usually included in routine diagnoses.

Keywords : Dairy cattle; Mycoplasma spp.

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