SciELO - Scientific Electronic Library Online

 
vol.33 número3Regional risk associated with the transport of hazardous materials índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Bookmark


Latin American applied research

versión impresa ISSN 0327-0793

Resumen

VISNOVSKY, G.; CLAUS, J. D.  y  MERCHUK, J. C.. Cultivation of insect cells in airlift reactors: influence of reactor configuration and superficial gas velocity. Lat. Am. appl. res. [online]. 2003, vol.33, n.3, pp. 207-211. ISSN 0327-0793.

Large-scale cultivation is an essential step towards the feasible production of baculovirus in insect cell cultures. Airlift reactors appear to offer considerable advantages over other insect cell culture systems. In order to evaluate the impact of reactor design on the behavior of insect cell cultures, the IPLB-Sf-21 cell line was cultivated in three different concentric tube airlift reactors that differ in their geometrical parameters. The ratio of downcomer to riser cross sectional areas, the shape of the bottom and the ratio of height to diameter of the reactor proved to be important since them produce significant differences on cell growth behavior. Modifying the reactor design the cellular growth rate could be improved from 0.016/h to 0.031/h, while the maximum viable cell density could be elevated from 9x105 to 2.4x106 cells/ml. Once selected a reactor configuration, the influence of gas flow rate was determined, finding an optimal value of superficial gas velocity that renders sufficient oxygenation without any significant effect on the cellular viability. In addition, the influence of the reactor design on fluid circulation in the reactor was tested.

Palabras llave : Insect Cell Culture; Airlift Reactor; Geometrical Parameters; Gas Flow Rate; Liquid Velocity.

        · texto en Inglés     · pdf en Inglés