SciELO - Scientific Electronic Library Online

 
vol.36 número4New material as support for nickel boride catalystThermodynamic approach for optimal design of heat and power plants: Relationships between thermodynamic and economics solutions índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Bookmark


Latin American applied research

versión impresa ISSN 0327-0793

Resumen

ZUQUELI, R.; PRIETO, C.; ETCHEVERRIGARAY, M.  y  KRATJE, R.. Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN-β production by mammalian cell culture. Lat. Am. appl. res. [online]. 2006, vol.36, n.4, pp. 321-327. ISSN 0327-0793.

Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater.

Palabras llave : rhIFN-β1a Production; CHO Cells; Zinc Sulphate; Sodium Butyrate.

        · texto en Inglés     · pdf en Inglés