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Latin American applied research

Print version ISSN 0327-0793


GHAREKHANI, M.; GHORBANI, M.  and  RASOULNEJAD, N.. Microwave-assisted extraction of phenolic and flavonoid compounds from Eucalyptus camaldulensis Dehn leaves as compared with ultrasound-assisted extraction. Lat. Am. appl. res. [online]. 2012, vol.42, n.3, pp.305-310. ISSN 0327-0793.

In the present study, a microwave-assisted extraction (MAE) technique has been developed for extraction of phenolic and flavonoid compounds from eucalyptus leaves. Various experimental conditions, such as solvent type, ethanol concentration, MAE time, liquid/solid ratio and pre-leaching time before MAE were examined to optimize the extraction. Among the solvents tested, 50% aqueous ethanol extracted the highest phenolic and flavonoid contents from eucalyptus leaves. The extraction of phenolic and flavonoid compounds with MAE for 5 min was equivalent with ultrasound-assisted extraction (UAE) (60 min) and traditional extraction (24 h) methods. Time of extraction at room temperature and UAE was about 288 and 12 times higher than the needed with MAE, respectively. Due to the considerable saving in time, MAE was more effective than the traditional and UAE methods.

Keywords : Microwave-Assisted Extraction; Eucalyptus Leaves; Total Phenolic Compounds; Total Flavonoid Compound.

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