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Latin American applied research

On-line version ISSN 1851-8796

Abstract

HERNANDEZ-LOPEZ, J.L.. High-sensitive bioactivity assay for hybrid nanostructured materials with plasmonic properties. Lat. Am. appl. res. [online]. 2014, vol.44, n.2, pp. 119-121. ISSN 1851-8796.

The binding of biotin to gold nanorods (Au-NRs) conjugated to neutravidin is accompanied by an increase in the fluorescence intensity of protein's tryptophan residues. Localized surface plasmon resonance (LSPR), combined with the known affinity of streptavidin for biotin, led to the development of a high-sensitive enzyme activity assay.

Keywords : Au-NRs/Neutravidin; LSPR; Enzyme Activity; In-vivo Imaging; Biological Sensing..

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