Abstract

DE CASTROPAZOS, Marcelo et al. Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation. Biocell [online]. 2003, vol.27, n.3, pp.301-309. ISSN 0327-9545.

The effects of Photodynamic Therapy using 2^nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2^nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm² (Power=26mW; l=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS₄₎ photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.

Keywords : Photodynamic therapy; Phthalocyanines; Laser; Cell death; Ultrastructure.

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