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ARNEODO, Joel; DE BREUIL, Soledad; LENARDON, Sergio  and  CONCI, Luis. Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I. Biocell [online]. 2005, vol.29, n.2, pp.205-207. ISSN 0327-9545.

Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.

Keywords : Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.

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