Resumen

PONTAROLI, A. C.  y  CAMADRO, E. L.. Plant regeneration after long term callus culture in clones of Asparagus officinalis L. Biocell [online]. 2005, vol.29, n.3, pp.313-317. ISSN 0327-9545.

Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l^-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l^-1 2,4-D and 1 mg.l^-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone ‘265' on MS medium with 5 mg.l^-1 2iP, 1 mg.l^-1 IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone ‘265' on MS medium with 0.1 mg.l^-1 kinetin, 0.1 mg.l^-1 NAA, 1.32 mg.l^-1 ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.

Palabras clave : Callus; Shoot primordia induction; Rooting; Medium x genotype interactions.

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