Abstract

PEREIRA, Marli Aparecida Dos Santos; BAGATIN, Maria Claúdia  and  ZANONI, Jacqueline Nelisis. Effects of the ascorbic acid supplementation on NADH-diaphorase myenteric neurons in the duodenum of diabetic rats. Biocell [online]. 2006, vol.30, n.2, pp.295-300. ISSN 0327-9545.

We assessed the ascorbic acid (AA) supplementation on the myenteric neurons in the duodenum of rats. Fifteen rats with 90 days of age were divided into three groups: control (C), diabetics (D) and ascorbic acid treated diabetics (DA). After 120 days of daily treatment with AA, the duodenum was submitted to the NADH-diaphorase (NADH-d) histochemical technique, which allowed us to evaluate the neuronal density in an area of 8.96 mm² for each duodenum, and also to measure the cellular profile area of 500 neurons per group. The supplementation promoted an increase on AA levels. The neuronal density (p <0.05) was higher in the group DA when compared to group D. There were no significant differences in the neuronal areas, when we compared groups C (204 ± 16.5) and D (146.3 ± 35.84) to groups D and DA (184.5 ± 5.6) (p>0.05). The AA-supplementation avoided the density reduction of the NADHd myenteric neurons in the duodenum of diabetic rats.

Keywords : Diabetes mellitus; Ascorbic acid; Myenteric neurons.

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