Abstract

CURVETTO, Néstor; MARINANGELI, Pablo  and  MOCKEL, Gabriela. Hydrogen peroxide in micropropagation of Lilium: A comparison with a traditional methodology. Biocell [online]. 2006, vol.30, n.3, pp.497-500. ISSN 0327-9545.

The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H₂O₂) into the nutrient media as chemical sterilizer. A series of H₂O₂ concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits in all treatments, though it was higher in the H₂O₂ treatments (40%) compared to the traditional methodology (20%). There were not significant differences in the number of bulblets per explant, and at the end of the multiplication phase, bulblets from 0.02% H₂O₂ treatment had greater biomass than from other treatments, indicating a beneficial effect. These bulblets also had a higher relative growth ratio with respect to the traditional method when cultivated for an additional period and showed the highest average bulblet fresh weight. It is expected that this higher bulblet mass would result in better performance during ex vitro cultivation.

Keywords : Chemical sterilization; Lily; In vitro multiplication; Tissue culture.

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