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versão impressa ISSN 0327-9545


SILVA-BRIANO, Marcelo et al. Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona. Biocell [online]. 2007, vol.31, n.2, pp.225-228. ISSN 0327-9545.

Syntaxin-1 and 25-kDa Synaptosome-associated Protein (SNAP-25) are present in the plasma membrane of several different secretory cell types and are involved in the exocytosis process. In this work, the free-living amoeba Difflugia corona was studied in relation to ultrastructure, structural membrane proteins, and proteins such as Syntaxin-1 and SNAP-25. Our results obtained by scanning electron microscopy in the amoeba without its theca, showed many membrane projections and several pore-like structures. Using immunocytochemistry, we found structural proteins Syntaxin-1 and SNAP-25.

Palavras-chave : Amoeba; Membrane proteins; Pore-like; Theca; Ultrastructure.

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