SciELO - Scientific Electronic Library Online

vol.32 issue2Cell proliferation of the ileum intestinal mucosa of diabetic rats treated with ascorbic acidBehavior of mesenchymal stem cells stained with 4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links



Print version ISSN 0327-9545


NOVAK, Ivón Teresa  and  CABRAL, Humberto Ramón. Rosette formation by macrophages with adhered T lymphocytes is precluded by inhibitors of antigen processing and presentation. Biocell [online]. 2008, vol.32, n.2, pp.169-174. ISSN 0327-9545.

We had previously found in autologous human leukocyte cultures, in which dead neutrophils phagocytosis by macrophages occur, macrophages and T CD4 lymphocytes perform a selective cell-cell interaction showing many figures of either one, two or several T- lymphocytes adhering to a central macrophage were seen. Considering that antigen presentation would be necessary for the formation of these immune synapses, we attempted to block rosette formation (i.e., the formation of macrophage associations with at least three lymphocytes) by interfering with both antigen processing and presentation. Culture samples of autologous leukocytes from 7 healthy donors were subjected to either brefeldin A, chloroquine or to an anti- HLA DR antibody. Rosette formation was significantly inhibited in the treated samples (either with brefeldin A, chloroquine or the anti- HLA DR; ANOVA, p<0.001, as compared with the untreated controls). It is concluded that interference with antigen processing and presentation precludes the formation of these cellcell interactions.

Keywords : Autologous rosetting; Cultured human macrophages and T- lymphocytes; Brefeldin A; Chloroquine; Immune synapses.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License