Abstract

HONG, Samin; PARK, Kyoungsoo; KIM, Chan Yun  and  SEONG, Gong Je. Agmatine inhibits hypoxia-induced TNF-a release from cultured retinal ganglion cells. Biocell [online]. 2008, vol.32, n.2, pp.201-205. ISSN 0327-9545.

The effect of hypoxia on the release of tumor necrosis factor-a (TNF-a) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-a in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100μM agmatine. The expression levels of TNF-a and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-a production in RGCs. Agmatine significantly reduced TNF-a level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or agmatine treatment. Our results show that agmatine inhibits the TNF-a production of RGCs in hypoxic condition. These results demonstrate a possible neuroprotective mechanism for agmatine against hypoxic damage in RGCs.

Keywords : Retinal ganglion cell; Tumor necrosis factor-a; Hypoxia; Agmatine.

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