SciELO - Scientific Electronic Library Online

 
vol.33 issue3C2- and C4-position 17β-estradiol metabolites and their relation to breast cancerChanges in the corpora allata and epidermal proliferation along the fourth instar of the Chagas disease vector Triatoma infestans author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Biocell

Print version ISSN 0327-9545

Abstract

MARTIN, Maria Teresa et al. Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait. Biocell [online]. 2009, vol.33, n.3, pp.141-148. ISSN 0327-9545.

Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 μM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 μM. In addition to that, jasmonic acid was a stronger inhibitor than Ethephon in any of the tried combinations. There were no significant differences between the control treatment and the treatments with only 10 μM of jasmonic acid or Ethephon. However, 10 μM 6-benzylaminopurine induced bud formation. The different combinations of 6-benzylaminopurine with jasmonic acid and Ethephon showed that concentrations of 10 to 100 μM did not affect the number of induced buds. Jasmonic acid had an inhibitory effect which Ethephon only showed when combined with 100 μM of jasmonic acid and 10 μM of 6-benzylaminopurine. Three response groups were defined by cluster analysis: group 1 produced the greatest mean number of buds (4 to 5) and a mean epicotyl growth of 1 to 1.5 cm; group 2 produced 2 to 4 buds and a mean growth of 0.5 to 1.2 cm; group 3 produced only one bud and a mean epicotyl length of 1.2 to 2 cm.

Keywords : Micropropagation; Phytohormones; Plant growth regulators; 6-benzylaminopurine; In vitro plant culture.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License