Abstract

BARRADAS-DERMITZ, Dulce Ma. et al. Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production. Biocell [online]. 2010, vol.34, n.1, pp.1-6. ISSN 0327-9545.

Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 μM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 μM elicitor, sampling at 10 days (p < 0.01, 6.45 mg/L). This same line also had the highest biomass production (6.85 g/L, p < 0.01) at 10 days of culture but at the higher elicitor concentration of 10 mM. All three cell lines did not produce paclitaxel under experimental conditions used.

Keywords : Growth-associated metabolites; Methyl jasmonate; Paclitaxel precursor; Secondary metabolism; Suspension cell culture.

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