SciELO - Scientific Electronic Library Online

vol.34 issue1Taxus globosa S. cell lines: Initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel productionCobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links



Print version ISSN 0327-9545


VILA, Silvia; GONZALEZ, Ana; REY, Hebe  and  MROGINSKI, Luis. Effect of morphological heterogeneity of somatic embryos of Melia azedarach on conversion into plants. Biocell [online]. 2010, vol.34, n.1, pp.7-13. ISSN 0327-9545.

Embryogenic cultures were initiated from immature Melia azedarach (Meliaceae) zigotic embryos. Explants were induced on Murashige and Skoog (1962) medium with 4.54 μM thidiazuron or 0.45 μM dichlorophenoxyacetic acid. After 6 weeks of culture on induction medium, somatic embryos were categorized in four morphological classes based on the presence of single or fused embryos and if they remained united or not to the original explant; that were evaluated histologically. The somatic embryos of every category were transferred, in groups or individually, on a 1/4 MS medium. Bipolar embryos, the more typically normal ones, had well defined shoot and root apical meristems and produced single plants; subcultured individually their conversion was 28%, and subcultured in groups the conversion declined to 6.8%. Fused embryos subcultured in groups had only a 2.1% conversion and produced plants with fused stems. None conversion rate in the others classes was associated to poorly developed shoot and root meristematic areas or with their absence. The converted plants were acclimatized and transferred, in a mist, to soil, with an independent of the class 95% survival rate.

Keywords : Somatic embryo morphology; Conversion; Histology; Scanning electron microscopy.

        · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License