Abstract

LOPEZ, Silvia N. et al. Massive and simultaneous production of Ceratitis capitata (Diptera: Tephritidae) males and Diachasmimorpha longicaudata (Hymenoptera: Braconidae) parasitoids. Rev. Soc. Entomol. Argent. [online]. 2006, vol.65, n.3-4, pp.99-108. ISSN 0373-5680.

In the genetic sexing strain «Cast191», the females of Ceratitis capitata are homozygous for the mutation slow , slowing down their rate of development, and the males are heterozygous, having a normal rate of development. This feature made Cast191 capable of producing sterile males, on one hand, and parasitoids that are reared on the remaining larvae, on the other. The objective of the present work was to do this two thing simultaneously and at a larger scale than previously. Besides, in an attempt to improve malefemale separation under this conditions, a pulse of 15ºC was applied to first stage larva during 1 or 2 days; these larvae were later held either at 20ºC or, at 25ºC until pupation, both groups of pupae being then kept at 25ºC . The best separation between male and female, achieved rearing larvae at 20ºC without any pulse of 15ºC , was then used to evaluate the quality of the parasitoids Diachasmimorpha longicaudata reared on the larvae remaining after male separation. These larvae were exposed to the parasitoid under standard conditions. The rate of parasitism obtained was similar to the one found when parasitoids are exposed to wild strain, and the F1 sex rate was even more biased toward females. The potential use at larger scale of the Cast191 strain of C. capitata for producing sterile males and D. longicaudata parasitoids is discussed.

Keywords : Ceratitis capitata; Genetic sexing strains; Sterile insect technique; Parasitoids.

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