Abstract

ALFONSO VALDES, Ivonne; ZHURBENKO, Raisa; LOBAINA RODRIGUEZ, Tamara  and  RODRIGUEZ MARTINEZ, Claudio. Effect of a combination of nutrients and inhibitors of bacterial growth on the development of Salmonella. Salud(i)ciencia [online]. 2018, vol.23, n.2, pp.127-133. ISSN 1667-8682.

Abstract The selection of nutrient bases depends on the microorganism and the purpose of the medium. Inhibitors of bacterial growth are of great importance in selective and differential media and may interfere in the growth of the microorganism of interest. An adequate balance between promoter substances and inhibitors of bacterial growth is thus required, especially for bacteria that could be found either at very low concentrations or those subject to different stress conditions during storage of the samples containing them, such as Salmonella. The aim was to evaluate the effect of a combination of nutrients of different origins and inhibitors of grampositive bacteria on the development of Salmonella serotypes. 52 Salmonella strains and a representation of other bacteria and yeasts were selected. The nutritional capacity of the composition was determined by spectrophotometric technique formulating variants with mixtures of nutritive bases, and recording the increase in biomass. Recovery capacity and inhibition of two experimental variants with different inhibitors were quantitatively tested. The productivity of the final formulation was compared with a chromogenic medium (Oxoid, England). Salmonella showed abundant growth in the variants made with different nutrient combinations. Both experimental formulations showed their ability to recover microorganisms of interest. The final composition showed productivity values higher than 0.80 in both variants. The mixture of nutrient bases and bile salts as an inhibitor of growth of grampositive bacteria was an effective combination, capable of stimulating the growth of Salmonella genus when these bacteria are found at low concentration.

Keywords : chromogenic and fluorogenic diagnostician; salmonella; culture media; bacterial colonization; bacterial nutrition; bacterial inhibitors.

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