Abstract

RISSO, A. et al. Biological validation of the "in field" extraction method for fecal estradiol in the domestic cat: A preliminary report. InVet [online]. 2010, vol.12, n.1, pp.53-58. ISSN 1668-3498.

The objective of this article was to biologically validate the ¨field method¨ for estradiol fecal extraction in domestic felids by a comparative study of samples obtained in different stages of the estrous cycle. A total at of 40 fecal samples from 7 postpubertal female cats were included. The samples were collected in the estrous (n=22) and interestrous (n=18) stages (defined by behavior and vaginal cytology) during 12 months. The "field extraction method¨ was carried out and then 17ß estradiol was measured by radiommunoassay. Fecal estradiol concentration ranged from de 926 to 33320 pg/g wet weight. Mean ± SEM in estrous and interestrous samples were 11814±2171 pg/g and 3238±474 pg/g (p < 0.01), respectively. Longitudinal observation of estradiol values clearly indicated that peaks coincided with cytological and behavioral estrus.

Keywords : Fecal extraction; Steroid; Felid.

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