SciELO - Scientific Electronic Library Online

 
vol.13 número1Influencia de la edad de vacunación con la vacuna B. abortus S19 en la respuesta serológica inducida en ternerasContaminación de carne molida con cepas de Escherichia coli shigatoxigénico (STEC) provenientes de comercios minoristas de San Martín, Buenos Aires, categorizados según nivel socioeconómico índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Bookmark


InVet

versión On-line ISSN 1668-3498

Resumen

TAMIOZZO, P.J; LUCCHESI, P.M.A  y  AMBROGI, A. Genetic diversity of Mycoplasma hyopneumoniae in pig farms from Argentina. InVet [online]. 2011, vol.13, n.1, pp. 27-35. ISSN 1668-3498.

Mycoplasma hyopneumoniae is the etiological agent of porcine enzootic pneumonia (EP), one of the respiratory diseases with higher impact on the swine industry. Knowledge of M. hyopneumoniae genetic diversity is useful for epidemiological and pathogenesis studies to develop effective control strategies. In this work, 39 DNA samples from nasal swabs and bronchial lavages, that previously rendered positive for this pathogen by nPCR, were analyzed. They corresponded to pigs of different ages, belonging to seven farms. For M. hyopneumoniae typing using DNA from nasal swabs, a nPCR assay was developed to amplify the region coding for the serine tandem repeat domain of p146 gene. For samples from bronchial lavages, the same region was amplified by PCR, along with other VNTRs (variable number tandem repeats) from other loci. With this approach, that proved to be useful for the direct analysis of clinical samples without prior isolation of the pathogen, the genetic diversity of M. hyopneumoniae strains circulating in our country could be evidenced. Different genotypes were found among farms and also among animals from a same farm, but there were also animals (from a different or a same farm) that harbored a same M. hyopneumoniae genotype.

Palabras llave : Mycoplasma hyopneumoniae; VNTR; P146gene; Typing.

        · resumen en Español     · texto en Español     · pdf en Español