On-line version ISSN 1668-3498
BERNARDI, S.F. et al. Evaluation of changes ocassioned by changes in bovine sperm in the management of dose during handling artificial insemination. InVet [online]. 2011, vol.13, n.2, pp. 25-38. ISSN 1668-3498.
The aim of this work was to determine the effect of the changes that doses undergo during thawing and before insemination due to a dissimilar handling of them. Focus was placed on the effects on various morphological and functional characteristics of spermatozoa which affect the quality of the semen to be inseminated. Samples from an Holando Argentine bull were employed, processed and frozen in 0.5cc straws using a semidefined diluent. Sperm motility was performed with Sperm Vision, plasmatic membrane functionality was tested with a HOST test and acrosome integrity under phase contrast microscope. Protocol changes that led to an unacceptable quality for insemination -according to ISO 9002- were related to abrupt thawing temperature changes and immersion time as well as to delays in insemination once a dose was thawed and in removing the Straw from the nitrogen flask. This same situation was observed when analysing the functionality of the plasmatic membrane. Acrosome integrity was higher when thawed at high temperatures (55, 75 and 95°C).
Keywords : Motility; Plasma membrane; Acrosome; Morphology; Bovine semen.