On-line version ISSN 1668-3498
DOUMECQ, M.L.; SOTO, P; ECHEVARRIA, H and MONTEAVARO, C. Cloned strains of Tritrichomonas foetus obtained from natural infections in cattle. InVet [online]. 2011, vol.13, n.2, pp. 61-67. ISSN 1668-3498.
The aim of this work is to describe a simple technique to cloned strain of Tritrichomonas foetus in a solid medium. From different strains from natural infections in cattle two techniques: Homogenized in tube and Homogenized in plate were tested. The must appropriate agar concentrations in the culture medium and incubation time were evaluated for each techniques. The results showed that the most appropriate technique was Homogenized in plate with an agar concentration of 0.45 g% w /v and an incubation time of 5 days. This technique is a useful and easy to obtain clones of T. foetus, allows the growth of protozoa in isolated colonies on solid media. Furthermore the technique can be used to evaluate the production of hemolytic colonies for the presence of bovine red blood cells in the culture medium.
Keywords : Tritrichomonas foetus; Cloned; Field strains isolates; Culture medium.