Resumen

GUZMAN, M. et al. Survival and infective capability of cryopreserved infective larvae of trichostongyles. InVet [online]. 2012, vol.14, n.1, pp.25-31. ISSN 1668-3498.

Cryopreservation of gastrointestinal nematode infective larvae (L3) has been used to preserver gastrointestinal nematodes in laboratory for a long time. The aim of this study was to establish the survival and infective capability of infective larvae after cryopreservation. A total of 2 isolates named as Ayacucho and Balcarce were used and L3 were exsheathed and thereafter, frozen in liquid nitrogen. Rate of survival (%) was estimated taking into account larval motility after thawing as discriminate factor for live/dead at 3 and 7 months from freezing. Six lambs were infected orally with L3 previously cryopreserved. The nematode infection was monitored through egg excretion in faeces. The rate of survival was 31-70% at 3 months and 68-72% at 7 months of freezing. Nematode eggs in faeces samples were detected at 3 weeks after infection being recorded no differences among isolates and/or freezing period of time. Advantages of the cryopreservation in nematodes research are emphasizing.

Palabras clave : Criopreservation; Haemonchus; Sheep; Thawing; Infection.

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